Altering of genes can lead to malformations of organs in babies: Research

Bonn [Germany], November 16 (ANI): The gene is the fundamental unit of any living creature. It also has a major role in the development of a human baby at the embryonic stage. A team of German researchers from the University of Bonn have revealed that any artificial alteration in it can have far-reaching consequences which can even result in malformations of certain organs when a baby is born.
According to a study published in the ‘Journal of Medical, the gene emerged very early in evolution. It also exists in zebrafish, for example, and performs a similar function there as it does in humans. Hence, any mutilations into its prior orientation can be fatal for long-term developments in the human embryo.
The researchers tracked down the gene when they studied two individuals with congenital malformations. “It was a man and his niece,” explains Dr. Gabriel Dworschak. “Both had malformed kidneys, urinary tract and oesophagus, and the man also had a malformed right arm and heart.”
The physician at the University Children’s Hospital in Bonn conducts research on rare genetic diseases at the Institutes of Anatomy and Human Genetics. When the team looked at the genetic makeup of the family members, they came across an anomaly: A gene called SHROOM4 was altered in affected individuals compared to healthy individuals.
SHROOM4 was already familiar from another context: It was known to play a key role in brain function. Mutations can result in intellectual impairment, epileptic seizures and behavioral abnormalities. “Our findings indicated though, that it may play a broader role in embryonic organ development,” Dworschak explains.
The team from Bonn searched internationally for other cases in which abnormalities in the SHROOM4 gene had also been found — and succeeded: “Together with our cooperation partners, this led us to four more affected individuals from three families,” says Prof. Dr. Heiko Reutter, who has since moved from the University Hospital Bonn to the University of Erlangen-Nuremberg. “All of them had the SHROOM4 gene altered, but not always in the same way.”
However, this did not necessarily clarify whether SHROOM4 variants were actually responsible for the malformations. But there is an animal that has a very similar gene: the zebrafish. It serves as a model organism in many genetic studies today — and not only because it is easy to keep in a species-appropriate manner and to reproduce quickly: The skin of its larvae is almost transparent. This makes it easy to observe the animals’ embryonic development under the light microscope. “Here at the University Hospital, we have the advantage that the research group led by Prof. Dr. Benjamin Odermatt from the Institute of Neuroanatomy works a lot with zebrafish,” stresses Dr. Caroline Kolvenbach, who was also involved in the study of SHROOM4. “This expertise came in handy in our study.”
The researchers almost completely inactivated SHROOM4 in the larvae. The animals then showed malformations similar to those seen in the patients. If, on the other hand, larvae with SHROOM4 switched off were injected with the intact human genetic material, they developed almost normally. “This shows first that they absolutely need a functional SHROOM4 for healthy development; and second, that the human gene can still take over the function of the fish gene,” Dworschak emphasizes.
The team now wants to find out which part the gene plays in embryonic development. “We assume that it is needed for very basic processes in the cell,” says Dworschak. “It’s hard to explain otherwise why changes in the same gene cause such a variety of symptoms.”
How a mouse, a dog or a human being develops from a fertilized ovum is still not fully understood. This is because the ovum has the ability to form any type of tissue in the organism, whether it is bone, skin, muscle or the brain. Its daughter cells are genetically identical to it; so in principle they should be able to do the same. But at a very early stage, certain programs are activated in their cells that irrevocably determine their developmental fate.
This process must be coordinated down to the finest detail. Because only then it is ensured that the eyes form in the appropriate spot on the face, while other cells very close by differentiate into the nasal cartilage. Surprisingly, however, there is no conductor wielding the baton. It is as if a Lego spaceship were assembling itself — only infinitely more complicated. “Our study is a small piece of the mosaic to this picture, which is still largely incomplete,” Dworschak says. (ANI)